Gabapentin served as a positive control in this study.The treatment of U-87 cells with LPS resulted into a marked increase in the cell viability.LPS pre-treated (500 ng/ml for 20 min) cells were treated with various concentrations of RT before the estimation of cytokines. 50 ± 6.6 μg/mg of protein; P The CCI induced a notable rise in the levels of pro-inflammatory cytokines.(A–D) Are the graphical representation of optical density of cell supernatants at 405 nm for TNF-α, IL-1β, IL-6 and IL-10. The tissue levels of TNF-α, IL-6 and IL-1β in the CCI-induced group were 56 ± 2.6, 44 ± 4.8 and 64 ± 6.8 pg/mg respectively.p The CCI induced characteristic histological changes in sciatic nerve.Alterations like nerve fibre swelling, inflammatory cell infilteration, fibre derangement and activation of neuroglial cell like satellite cells and Schwann cells suggest the damage of the sciatic nerve.Whereas, treatment of cell with RT at tested concentrations decreased LPS-mediated ROS induced cell viability in a dose dependent manner (Fig. There was no considerable cytotoxicity due to RT treatment in absence of LPS stimulation (data not shown).To overrule the interference of cytotoxicity of RT, we tested the concentrations of RT which were devoid of any prominent effect on the cell viability.
The need to discover novel treatment modalities for neuropathic conditions still prevails.
Rhus Tox decreased oxidative stress and cytokine release with restoration of anti-oxidant systems.
Chronic treatment with Rhus Tox ultra dilutions for 14 days ameliorated neuropathic pain revealed as inhibition of cold, warm and mechanical allodynia along with improved motor nerve conduction velocity (MNCV) in constricted nerve.
Toxicodendron pubescens, popularly known as Rhus Tox (RT) is recommended in alternative medicines as an anti-inflammatory and analgesic remedy.
Earlier, we reported anti-inflammatory, anti-arthritic and immunomodulatory activities of Rhus Tox.